Kitazato media’s composition is entirely synthetic. Among their components, the following are notable:
✓ Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the survival rate in hatched blastocysts and reduces mechanical stress during warming.
✓ Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.
✓ DMSO in combination with ethylene glycol in the media assures less toxicity and the best outcomes after warming.
✓ Identification marks to define the side where specimens should be loaded for quick location of the cryopreserved samples during thawing
✓ The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions.
The Cryotop® Method offers the same products for both oocyte and embryo vitrification. This helps to standardize procedures, optimize laboratory routine and improve clinical results.